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Protein bands were visualized with an ECL solution (Thermo Fisher Scientific) and imaged with a ChemiDoc Imaging System (BIO-RAD)

Current approaches to vaccinology largely rely on trial and error, with immunogenic vaccines progressing to increasingly large human trials to determine their efficacy

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Despite their overlapping features, the underlying pathophysiological mechanisms of abdominal pain in FC remain poorly understood, and it is unclear whether the pain mechanisms in FC resemble those in IBS-C or arise from distinct processes [16, 17]